diff --git a/backend/ulozto/ulozto.go b/backend/ulozto/ulozto.go
index cf92de32a..44810f310 100644
--- a/backend/ulozto/ulozto.go
+++ b/backend/ulozto/ulozto.go
@@ -38,8 +38,6 @@ const (
 	maxSleep      = 2 * time.Second
 	decayConstant = 2 // bigger for slower decay, exponential
 	rootURL       = "https://apis.uloz.to"
-	// TODO temporary limitation, remove with chunked upload impl
-	maxFileSizeBytes = 2500 * 1024 * 1024
 )
 
 // Options defines the configuration for this backend
@@ -194,9 +192,7 @@ func errorHandler(resp *http.Response) error {
 // retryErrorCodes is a slice of error codes that we will retry
 var retryErrorCodes = []int{
 	429, // Too Many Requests.
-	// TODO: random 500s should be retried but the error code corresponds to a known issue with uploading large files,
-	//   leading to numerous (slow & resource consuming) retries. Don't retry them until the root cause is addressed.
-	// 500, // Internal Server Error
+	500, // Internal Server Error
 	502, // Bad Gateway
 	503, // Service Unavailable
 	504, // Gateway Timeout
@@ -447,10 +443,6 @@ func (f *Fs) uploadUnchecked(ctx context.Context, name, parentSlug string, info
 
 // Put implements the mandatory method fs.Fs.Put.
 func (f *Fs) Put(ctx context.Context, in io.Reader, src fs.ObjectInfo, options ...fs.OpenOption) (fs.Object, error) {
-	// TODO: workaround for uloz.to's bug. Remove when chunked upload support is implemented.
-	if src.Size() > maxFileSizeBytes {
-		return nil, errors.New("file size over the supported max threshold")
-	}
 	existingObj, err := f.NewObject(ctx, src.Remote())
 
 	switch {
diff --git a/backend/ulozto/ulozto_test.go b/backend/ulozto/ulozto_test.go
index 99507fc24..8a7a54173 100644
--- a/backend/ulozto/ulozto_test.go
+++ b/backend/ulozto/ulozto_test.go
@@ -1,7 +1,6 @@
 package ulozto
 
 import (
-	"bytes"
 	"context"
 	"errors"
 	"testing"
@@ -10,7 +9,6 @@ import (
 	"github.com/rclone/rclone/backend/ulozto/api"
 	"github.com/rclone/rclone/fs"
 	"github.com/rclone/rclone/fs/hash"
-	"github.com/rclone/rclone/fs/object"
 	"github.com/rclone/rclone/fs/operations"
 	"github.com/rclone/rclone/fstest"
 	"github.com/stretchr/testify/require"
@@ -86,32 +84,3 @@ func TestListWithoutMetadata(t *testing.T) {
 	// Tear down
 	require.NoError(t, operations.Purge(ctx, f, ""))
 }
-
-// TestUploadLargeFile verifies that files over the supported threshold are not uploaded.
-func TestUploadLargeFile(t *testing.T) {
-	const (
-		remoteName = "TestUlozto:"
-		payload    = "foobar"
-		filesize   = maxFileSizeBytes + 1
-	)
-	ctx := context.Background()
-	fstest.Initialise()
-	subRemoteName, subRemoteLeaf, err := fstest.RandomRemoteName(remoteName)
-	require.NoError(t, err)
-	f, err := fs.NewFs(ctx, subRemoteName)
-	if errors.Is(err, fs.ErrorNotFoundInConfigFile) {
-		t.Logf("Didn't find %q in config file - skipping tests", remoteName)
-		return
-	}
-	require.NoError(t, err)
-
-	file := fstest.Item{ModTime: time.UnixMilli(123456789), Path: subRemoteLeaf, Size: int64(filesize)}
-	obji := object.NewStaticObjectInfo(file.Path, file.ModTime, file.Size, true, nil, nil)
-	// The payload buffer is just a placeholder which shouldn't be used
-	_, err = f.Put(ctx, bytes.NewBufferString(payload), obji)
-
-	require.Error(t, err, "File size over the supported max threshold.")
-
-	// Verify the remote stayed intact
-	fstest.CheckListing(t, f, []fstest.Item{})
-}